摘要:[Objectives] This study was conducted to explore the optimal period of TMV control and the physiological sites that interfere with TMV infection.[Methods] Proteome analysis was performed on the host tissues (tobacco plants) at different time periods of viral infection, to verify the changes in the expression of differential protein genes and N and PR1-a in the photosynthetic pathway and porphyrin metabolism and chlorophyll metabolism pathways in proteome;and the tobacco plants were treated with different preparations.[Results] The expression levels of N and PR1-a in the tobacco leaves treated with preparation B reached the highest level, and the effects on the expression levels of the differential protein genes were also the most significant. The control effects of the preparations were tested by the half-leaf method, and the results showed that preparation B had a significant control effect against the early infection of the virus.[Conclusions] This study lays a foundation for the prevention and control of tobacco mosaic virus on crops.
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主管单位:黑龙江省科学院;主办单位:黑龙江省科学院微生物研究所;黑龙江省微生物学会;黑龙江省生物工程学会